A team of researchers from the Institut Vision in Paris and the Jean-Pierre Aubert Research Centre, under the direction of Alain Chedotal, have managed to map the human embryo in three dimensions (see HERE the published study), which, they believe, permit better understanding of the mechanisms of formation of embryonic organs in normal and pathological conditions. Until this technique was developed, 3D embryonic reconstructions were obtained from thousands of embryos and fetuses, in which microscopic sections were cut at different stages of development. However, this new technique enables the inside of the entire embryo to be seen during the first trimester of its life. To that end, the researchers labeled the cells that they wanted to study with fluorescent proteins, and then made the embryo transparent by immersing it in different solvents, which removed its membranes, but conserved its protein structure; the embryo was then scanned under fluorescence microscopy. Using this technique, they analyzed embryos and fetuses from 6 to 14 weeks of gestation, constructing a three-dimensional atlas of the human embryo that can be used for both teachings and possibly for experimental techniques. There is no need to highlight the great ethical difficulties with these techniques, as they destroy human embryos with no further consideration. Therefore, although the results obtained are experimentally positive, the method used ethically disqualifies the overall process.
Photo: A view of an embryonic lung with this technique by Inserm